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Confocal Microscopy

Home > Microscopy > Confocal Microscopy

NEWS: We have recently added live-cell confocal imaging to our CMIF instrumentation.
The new instrument is a Visitech Infinity3 and is housed in our facility on the second
floor of the Biomedical Research Towers on W 12th avenue accross from the
Heart and Lung Facility.
If you are interested in Live-Cell Confocal imaging please feel free to contact the CMIF Director Dr. Burry to discuss your confocal projects.


Confocal laser scanning microscopy permits high resolution optical sectioning without compromising specimen integrity. Some advantages of using confocal microscopy include:

  • Elimination of out of focus planes, yielding sharper images than conventional fluorescence microscopy.
  • Analysis of thick specimens.
  • Computer controlled microscopy, permitting fine focus control and digitization of images.
  • Collection of data sets for subsequent three dimensional reconstruction.
  • Ability to collect two images simultaneously from specimens labelled with multiple fluorochromes.

Sample Images

Slice of pollen Another slice of pollen
Single confocal image
of pollen grain.
Another image deeper
into the specimen.
projection of pollen slices 3d view of pollen.
Projection made from
multiple thin sections.
Red/green stereo image.
View with standard red/green
3D glasses.
Single confocal image
of zoea insect.
Another image deeper
into the specimen.
Projection made from
multiple thin sections.
Red/green stereo image.
View with standard red/green
3D glasses.

Sample Applications

  • Distribution of drug on polymer delivery system.
  • Pseudopod extension during phagocytosis.
  • Tyrosine Kinase signal distribution following receptor stimulation.

Instrumentation

The Olympus Flowview 1000 Laser Scanning Confocal microscopes are equipped with:

  • Olympus upright microscope 10X, 20X, 40X,and 60X objectives and DIC optics
  • Lasers include HeNe1 (543 nm) , HeNe2 (633 nm), Argon/2 laser (458,477,488,514 nm), and 405nm Diode laser suitable for DAPI.
  • Spectral detector system for a finer separation of fluorochromes (FV1000-Spectra)
  • A second work station with a full version of software for image manipulation

    Zeiss 510 META Confocal

Training is available, please call or email us:
Phone 614-292-9786
FAX 614-688-8742
Or use the CMIF Contact Form to send a message direclty to the staff.


NEWS:  We have added two-photon confocal imaging to our
instrumentation. We now have two single-photon Olympus FV1000 confocals.
This new instrumentation is housed in our facility on the second floor of the Biomedical Research Towers on W 12th avenue accross from the Heart and Lung Facility.
If you are interested in Live-Cell or two-photon Confocal imaging please feel free to contact our CMIF Director Dr. Burry to discuss your confocal projects.