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Campus Microscopy & Imaging Facility : The Ohio State University
http://medicine.osu.edu/cmif//cmif/416.cfm

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Transmission Electron Microscopy

Home > Microscopy > Transmission Electron Microscopy

The CMIF is organized to facilitate the examination of a broad range of specimens from cell cultures to polymers to botanical samples. Cryo-sectioning and TEM examination at liquid nitrogen temperature is an option. Services provided by the CMIF staff can be tailored to the needs of the investigator. In addition to actual microscope use, the CMIF can provide specimen preparation, with special expertise in negative staining, immunocytochemistry, cryo-sectioning, and ultramicrotomy of polymer samples.

Sample Images

TEM of a neutrophil
Immunocytochemical gold
stain of cell division
SEM of fish sperm
TEM of insect muscle

Sample Applications

  • Localization of lysozyme in horse Paneth cells treated with different drugs.
  • Identification of plant virus using negative staining.
  • Changes in cardiac muscle mitochondria with copper deficiency.
  • Changes in distribution of microtubules at different stages of cell division.

Instrumentation

  • FEI Technai G2 Spirit Transmission Electron Microscope (TEM)

A Technai G2 Spirit is available for transmission electron microscopy.  It has the  capabilities of using kV's from 20 - 120, is high resolution, digital, and easy to use. This microscope has cryo-capability with the ability to image samples at liquid nitrogen temperature. Thin films of biological and material samples may be flash-frozen and examined, or sections of frozen material may be obtained using the Leica EM UC6 Ultramicrotome equipped with an FC6 cryo unit.

Technai G2 Spirit Biotwin TEM