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Welcome!

Not sure where to begin in order to start using CMIF/MSR? Please refer to this guide.

News and Announcements

CMIF wins Nikon Small World Prize!

Ticks are external parasites. They are widely distributed around the world, especially in warm, humid environment. Ticks live by sucking the blood as their nutrition. Because of their blood-ingesting diets and infections caused by pathogens, ticks act as vectors of many serious diseases that affect humans and other animals.  
 
Dr. Tong Zhang imaged this sample by taking the advantage of sample auto-fluorescence under different wavelengths with a 10x objective on a Nikon A1R Confocal Microscope at CMIF. There were 423 Z-direction images covering through a 211um thickness. Extended focus function re-built the 3D Z-stack images into a 2D image with 3D information. The microscope resonant scanner boosted the acquisition speed significantly.

This image had very fine details and striking looking. The auto-fluorescence revealed tick head’s anatomy and specially the mouth region with inverted-arrow-like structure. Ticks use this kind of structure to anchor them on animals. It is a good example of nature smart designs.

The Nikon Small World Competition first began in 1975 as a means to recognize and applaud the efforts of those involved with photography through the light microscope. Since then, its has become a leading showcase for photomicrographers from the widest array of scientific disciplines. This year is the 47th year of the competition, which had almost 1,900 images from 88 countries submitted for consideration.

https://www.nikonsmallworld.com/galleries/2021-photomicrography-competi…

Ticket Head
Tong Zhang, PhD and Paul Stoodley, PhD, Campus Microscopy & Imaging Facility (CMIF)
Winning the 7th Place in 2021 Nikon Small World Photomicrography Competition

Announcing Nikon Super-resolution Microscope at CMIF/MSR!

Super-resolution


The Nikon Super-resolution Microscope system is now installed in the newly renovated BSL2 space on the 2nd floor of the Biomedical Research Tower (BRT) at Campus Microscopy and Imaging Facility (CMIF) and Microscopy Shared Resource (MSR). The system is consist of Structured Illumination Microscopy (SIM), STochastic Optical Reconstruction Microscopy (STORM) and Total Internal Reflection Fluorescence (TIRF) microscopy capabilities. The SIM and TIRF can be used with a range of commonly used fluorophores including expressed fluorescent proteins. The SIM can resolve up to 115nm in 3D-SIM mode and 86nm in TIRF-SIM mode in the X/Y plane. The STORM offers down to 20nm resolution in the X/Y plane and 50nm in the Z plane, but it requires specialized probes and optimization. This is a Widefield based system and unlike the confocals which have multiple detectors, multiple color images are built up by rapidly capturing the signals from each fluorophore sequentially. This is a $750,000 instrument, which was funded by the NIH S10 grant, with supporting funds from the OSU Comprehensive Cancer Center (OSUCCC) for the STORM capability and the Office of Research for the room remodeling.

 
Dr. Tong Zhang, the Associate Director at CMIF/MSR can help you for questions about this system and provide trainings. We have strived to make the system with affordable rate at $45/hr or $360/day, which is lower than the average rate for comparable Big 10 and regional academic institution microscopy cores. Dr. Anthony Vetter from Nikon has agreed to spend one afternoon each month at the CMIF/MSR to discuss applications and operations on this system. Please let us know if you would like to take advantage of this opportunity. The CMIF/MSR staff are happy to provide tours and trainings as well. In addition, we have added a 7 lasers Olympus FV3000 system to our confocal portfolios.

We hope to see you soon and help your advancement in research with our services. 

Please don't forget to acknowledge the CMIF/MSR and NIH S10 grant in your publications:
We acknowledge resources from the Campus Microscopy and Imaging Facility (CMIF), and the OSU Comprehensive Cancer Center (OSUCCC) Microscopy Shared Resource (MSR) at the Ohio State University with NIH S10 OD025008 and NIH NIC P30CA016058.
 

 


To Nikon A1R Users:

The software has been updated on Feb. 5, 2021.

To make the software looking as similar as the old version and make sure all functions work properly, please note the new username/password are listed in Nikon A1R room.

Please watch this video (below) for the comparison between the old and new version software (Notice: this is only for the reference purpose, some details are not identical).

(YouTube - Created by University of Pittsburgh Center for Biologic Imaging)

We are working on a new version manual. It will be available to all users soon.

--CMIF, Feb. 9, 2021


WORKDAY ANNOUCMENT

On 1/7/2020 our scheduling system (FOM.osu.edu) that is used for making reservations for our instrumentation was integrated into Workday. All users will need to have a Workday requisition that has been entered into FOM.

For directions on how to create a requisition in Workday that will be used in FOM, please refer to this KB article. Please make sure to select "CMIF - Campus Microscopy Imaging Facility" as the supplier.


Imaging Beyond the Diffraction Limit: Nikon N-SIM and N-STORM Super-resolution Systems coming to CMIF/MSR soon

Excuse the disruption as we remodel to house the system in the BRT268 BSL2 suite!

Imaging Beyond the Diffraction Limit