The CMIF and the Chronic Brain Injury Program are happy to announce the acquisition of a Miltenyi UltraMicroscope Blaze light sheet microscope to be housed in room 245A of CMIF in the BRT. The new light sheet microscope has arrived and is scheduled to be installed in late January or early February 2026. This scope is ideal for cleared tissue or organoids. A tissue-clearing workshop and seminar will be announced soon. More details to come! 

Miltenyi UltraMicroscope Blaze Light Sheet Microscope

Introducing the Miltenyi UltraMicroscope Blaze light sheet microscope 

The Blaze is ideal for large, cleared samples. 

The main benefits are:

  • Multiple light sheets optimized for uniform illumination
  • Automated features for smooth transition between samples and objective switching
  • Sample holders tailored to your needs, including chambers to image multiple rodent organs or organoids up to an entire mouse
  • Easy adaptation to different clearing solutions
  • High-resolution to view sub-cellular details

The specifications are as follows:

  • Light sheet thickness: <4.5 μm @488 nm
  • Focus range: >±5mm from center position of sample for all imaging solutions
  • Motorized detection module for up to three MI Plan objective lenses
    • Motorized functions: z-drive, autofocus, filter wheel for up to seven 43 mm emission
  • Objective lenses of 1.1x, 4x, and 12x
  • magnification change unit (0.6x,1x,1.67x and 2.5x), so magnification from 0.66x to 30x
  • Motorized XYZ-stage for moving the cuvette and the sample holder (travel range 24 x

                   50 x 23 mm xyz)

  • A motorized cuvette release enables easy sample access
  • Standard Cuvette with 497 ml capacity and a XXL Chamber with 715 ml capacity
  • 4.2 Mpx camera:
    • 2048 x 2048 pixels
    • 6.5 μm pixel size
    • QE: 82% @Peak
  • Laser Beam Combiner equipped with following laser modules and emission filters:
    • 405 nm  75 mW, EMF460/40
    • 488 nm  75 mW, EMF525/50
    • 561 nm  75 mW, EMF620/60
    • 640 nm  65 mW, EMF680/30
    • 785 nm  75 mW, EMF805LP

 

Funding for the Miltenyi UltraMicroscope Blaze was provided by the Chronic Brain Injury Program.